Effect of dependent scattering on the optical properties of Intralipid tissue phantoms
نویسندگان
چکیده
The calibration of optical tissue-simulating phantoms remains an open question in spite of the many techniques proposed for accurate measurements of optical properties. As a consequence, a reference phantom with well known optical properties is still missing. As a first step towards a reference phantom we have recently proposed to use dilutions of Intralipid 20%. In this paper we discuss a matter that is commonly ignored when dilutions are prepared, i.e., the possibility of deviations from the simple linear relationships between the optical properties of the dilution and the Intralipid concentration due to the effects of dependent scattering. The results of an experimental investigation showed that dependent scattering does not affect absorption. As for the reduced scattering coefficient the effect can be described adding a term proportional to the square of the concentration. However, for concentrations of interest for tissue optics deviations from linearity remain within about 2%. The experimental investigation also showed that the microphysical properties of Intralipid are not affected by dilution. These results show the possibility to easily obtain a liquid diffusive phantom whose optical properties are known with error smaller than about 1%. Due to the intrinsic limitations of the different techniques proposed for measuring the optical properties it seems difficult to obtain a similar accuracy for solid phantoms.
منابع مشابه
Frequency-modulated light scattering interferometry employed for optical properties and dynamics studies of turbid media
In the present work, fiber-based frequency-modulated light scattering interferometry (FMLSI) is developed and employed for studies of optical properties and dynamics in liquid phantoms made from Intralipid(®). The fiber-based FMLSI system retrieves the optical properties by examining the intensity fluctuations through the turbid medium in a heterodyne detection scheme using a continuous-wave fr...
متن کاملDependence of optical scattering from Intralipid in gelatin-gel based tissue-mimicking phantoms on mixing temperature and time.
Intralipid is widely used as an optical scattering agent in tissue-mimicking phantoms. Accurate control when using Intralipid is critical to match the optical diffusivity of phantoms to the prescribed value. Currently, most protocols of Intralipid-based hydrogel phantom fabrication focus on factors such as Intralipid brand and concentration. In this note, for the first time to our knowledge, we...
متن کاملCharacterization and standardization of tissue-simulating protoporphyrin IX optical phantoms.
Optical devices for measuring protoporphryin IX (PpIX) fluorescence in tissue are routinely validated by measurements in optical phantoms. Yet there exists limited data to form a consensus on the recipe for phantoms that both mimic the optical properties found in tissue and yield a reliable and stable relationship between PpIX concentration and the fluorescence remission intensity. This study c...
متن کاملDo different turbid media with matched bulk optical properties also exhibit similar polarization properties?
We here investigate polarimetric behavior of thick samples of porcine liver, Intralipid, and microsphere-based tissue phantoms whose absorption and scattering properties are matched. Using polarized light we measured reflection mode Mueller matrices and derived linear/circular/total depolarization rates, based on polar decomposition. According to our results, phantoms exhibit greater depolariza...
متن کاملEngineering of core/shell nanoparticles surface plasmon for increasing of light penetration depth in tissue (modeling and analysis)
Objectives: In this article, a new procedure for increasing the light penetration depth in a tissue is studied and simulated. It has been reported that the most important problem in biomedical optical imaging relates to the light penetration depth, and so this makes a dramatic restriction on its applications. In the optical imaging method, the detection of the backscattered pho...
متن کامل